Deutsch
 
Benutzerhandbuch Datenschutzhinweis Impressum Kontakt
  DetailsucheBrowse

Datensatz

DATENSATZ AKTIONENEXPORT

Freigegeben

Zeitschriftenartikel

Crosstalk of cardiomyocytes and fibroblasts in co-cultures

MPG-Autoren
/persons/resource/persons188793

Richter,  Claudia
Research Group Biomedical Physics, Max Planck Institute for Dynamics and Self-Organization, Max Planck Society;

/persons/resource/persons188801

Turco,  Laura
Laboratory for Fluid Dynamics, Pattern Formation and Biocomplexity, Max Planck Institute for Dynamics and Self-Organization, Max Planck Society;

/persons/resource/persons41415

Knoch,  Fabian
Laboratory for Fluid Dynamics, Pattern Formation and Biocomplexity, Max Planck Institute for Dynamics and Self-Organization, Max Planck Society;

/persons/resource/persons173583

Luther,  Stefan
Research Group Biomedical Physics, Max Planck Institute for Dynamics and Self-Organization, Max Planck Society;

/persons/resource/persons173472

Bodenschatz,  Eberhard
Laboratory for Fluid Dynamics, Pattern Formation and Biocomplexity, Max Planck Institute for Dynamics and Self-Organization, Max Planck Society;

/persons/resource/persons173681

Tarantola,  Marco
Laboratory for Fluid Dynamics, Pattern Formation and Biocomplexity, Max Planck Institute for Dynamics and Self-Organization, Max Planck Society;

Externe Ressourcen
Volltexte (frei zugänglich)
Es sind keine frei zugänglichen Volltexte verfügbar
Ergänzendes Material (frei zugänglich)
Es sind keine frei zugänglichen Ergänzenden Materialien verfügbar
Zitation

Rother, J., Richter, C., Turco, L., Knoch, F., Mey, I., Luther, S., et al. (2015). Crosstalk of cardiomyocytes and fibroblasts in co-cultures. Open Biology, 5(6): 150038. doi:10.1098/rsob.150038.


Zitierlink: http://hdl.handle.net/11858/00-001M-0000-0029-7B2E-B
Zusammenfassung
Electromechanical function of cardiac muscle depends critically on the crosstalk of myocytes with non-myocytes. Upon cardiac fibrosis, fibroblasts translocate into infarcted necrotic tissue and alter their communication capabilities. In the present in vitro study, we determined a multiple parameter space relevant for fibrotic cardiac tissue development comprising the following essential processes: (i) adhesion to substrates with varying elasticity, (ii) dynamics of contractile function, and (iii) electromechanical connectivity. By combining electric cell-substrate impedance sensing (ECIS) with conventional optical microscopy, we could measure the impact of fibroblast–cardiomyocyte ratio on the aforementioned parameters in a non-invasive fashion. Adhesion to electrodes was quantified via spreading rates derived from impedance changes, period analysis allowed us to measure contraction dynamics and modulations of the barrier resistance served as a measure of connectivity. In summary, we claim that: (i) a preferred window for substrate elasticity around 7 kPa for low fibroblast content exists, which is shifted to stiffer substrates with increasing fibroblast fractions. (ii) Beat frequency decreases nonlinearly with increasing fraction of fibroblasts, while (iii) the intercellular resistance increases with a maximal functional connectivity at 75% fibroblasts. For the first time, cardiac cell–cell junction density-dependent connectivity in co-cultures of cardiomyocytes and fibroblasts was quantified using ECIS.