Item

Abstract

Recently, a new and versatile assay to determine the partitioning
coef
fi
cient
K
P
as a measure for the af
fi
nity of peripheral membrane
proteins for lipid bilayers was presented in the research article enti-
tled,
“
Introducing a
fl
uorescence-based standard to quantify protein
partitioning into membranes
”
[1]
. Here, the well-characterized bind-
ing of hexahistidine-tag (His
6
) to NTA(Ni) was utilized. Com-
plementarily, this data article reports the average diffusion coef
fi
cient
D
of His
6
-tagged enhanced green
fl
uorescent protein (eGFP-His
6
)and
the
fl
uorescent lipid analog ATTO
‐
647N
‐
DOPE in giant unilamellar
vesicles (GUVs) containing different amounts of NTA(Ni) lipids. In
addition, dissociation constants
K
d
of the NTA(Ni)/eGFP-His
6
system
are reported. Further, a conversion between
K
d
and
K
P
is provided