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Journal Article

Diffusion coefficients and dissociation constants of enhanced green fluorescent protein binding to free standing membranes

MPS-Authors
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Thomas,  Franziska A.
Schwille, Petra / Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, Max Planck Society;

/persons/resource/persons132982

Visco,  Ilaria
Schwille, Petra / Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, Max Planck Society;

/persons/resource/persons96518

Petrasek,  Zdenek
Schwille, Petra / Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, Max Planck Society;

/persons/resource/persons78084

Heinemann,  Fabian
Schwille, Petra / Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, Max Planck Society;

/persons/resource/persons15815

Schwille,  Petra
Schwille, Petra / Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, Max Planck Society;

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Fulltext (public)

1-s2.0-S2352340915002504-main.pdf
(Publisher version), 306KB

Supplementary Material (public)

1-s2.0-S2352340915002504-mmc1.zip
(Supplementary material), 6MB

Citation

Thomas, F. A., Visco, I., Petrasek, Z., Heinemann, F., & Schwille, P. (2015). Diffusion coefficients and dissociation constants of enhanced green fluorescent protein binding to free standing membranes. Data in Brief, 5, 537-541. doi:10.1016/j.dib.2015.10.002.


Cite as: http://hdl.handle.net/11858/00-001M-0000-0029-B204-D
Abstract
Recently, a new and versatile assay to determine the partitioning coef fi cient K P as a measure for the af fi nity of peripheral membrane proteins for lipid bilayers was presented in the research article enti- tled, “ Introducing a fl uorescence-based standard to quantify protein partitioning into membranes ” [1] . Here, the well-characterized bind- ing of hexahistidine-tag (His 6 ) to NTA(Ni) was utilized. Com- plementarily, this data article reports the average diffusion coef fi cient D of His 6 -tagged enhanced green fl uorescent protein (eGFP-His 6 )and the fl uorescent lipid analog ATTO ‐ 647N ‐ DOPE in giant unilamellar vesicles (GUVs) containing different amounts of NTA(Ni) lipids. In addition, dissociation constants K d of the NTA(Ni)/eGFP-His 6 system are reported. Further, a conversion between K d and K P is provided