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Volta phase plate cryo-EM of the small protein complex Prx3

MPS-Authors
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Khoshouei,  Maryam
Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society;

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Plitzko,  Jürgen M.
Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society;

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Baumeister,  Wolfgang
Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society;

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Danev,  Radostin
Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society;

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ncomms10534.pdf
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Citation

Khoshouei, M., Radjainia, M., Phillips, A. J., Gerrard, J. A., Mitra, A. K., Plitzko, J. M., et al. (2016). Volta phase plate cryo-EM of the small protein complex Prx3. NATURE COMMUNICATIONS, 7: 10534. doi:10.1038/ncomms10534.


Cite as: http://hdl.handle.net/11858/00-001M-0000-0029-C7F9-B
Abstract
Cryo-EM of large, macromolecular assemblies has seen a significant increase in the numbers of high-resolution structures since the arrival of direct electron detectors. However, sub-nanometre resolution cryo-EM structures are rare compared with crystal structure depositions, particularly for relatively small particles (<400 kDa). Here we demonstrate the benefits of Volta phase plates for single-particle analysis by time-efficient cryo-EM structure determination of 257 kDa human peroxiredoxin-3 dodecamers at 4.4 angstrom resolution. The Volta phase plate improves the applicability of cryo-EM for small molecules and accelerates structure determination.