English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT

Released

Journal Article

Miniaturized two-photon microscope based on a flexible coherent fiber bundle and a gradient-index lens objective

MPS-Authors
/persons/resource/persons93732

Kerr,  Jason
Department of Cell Physiology, Max Planck Institute for Medical Research, Max Planck Society;
Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons94557

Nimmerjahn,  Axel
Department of Cell Physiology, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons93373

Helmchen,  Fritjof
Department of Cell Physiology, Max Planck Institute for Medical Research, Max Planck Society;

Fulltext (public)
There are no public fulltexts available
Supplementary Material (public)
There is no public supplementary material available
Citation

Göbel, W., Kerr, J., Nimmerjahn, A., & Helmchen, F. (2004). Miniaturized two-photon microscope based on a flexible coherent fiber bundle and a gradient-index lens objective. Optics Letters, 29(21), 2521-2523. doi:10.1364/OL.29.002521.


Cite as: http://hdl.handle.net/11858/00-001M-0000-002A-18EF-6
Abstract
We present a miniature, flexible two-photon microscope consisting of a fused coherent optical fiber bundle with 30,000 cores and a gradient-index lens objective. The laser focus of a standard two-photon laser-scanning microscope was scanned over the entrance surface of the fiber bundle, resulting in sequential coupling into individual cores. Fluorescent light was detected through the fiber bundle. Micrometer-sized fluorescent beads and pollen grains were readily resolved. In addition, fluorescently labeled blood vessels were imaged through the fiber bundle in rat brain in vivo.