English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT

Released

Journal Article

Second-harmonic imaging of cornea after intrastromal femtosecond laser ablation

MPS-Authors
/persons/resource/persons118183

Giese,  Günter
Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society;

Fulltext (public)
There are no public fulltexts stored in PuRe
Supplementary Material (public)
There is no public supplementary material available
Citation

Han, M., Zickler, L., Giese, G., Walter, M., Loesel, F. H., & Bille, J. F. (2004). Second-harmonic imaging of cornea after intrastromal femtosecond laser ablation. Journal of Biomedical Optics, 9(4), 760-766. doi:10.1117/1.1756919.


Cite as: http://hdl.handle.net/11858/00-001M-0000-002A-1961-9
Abstract
Nonlinear laser scanning microscopy is widely used for noninvasive imaging in cell biology and tissue physiology. However, multiphoton fluorescence imaging of dense, transparent connective tissue (e.g., cornea) is challenging since sophisticated labeling or slicing is necessary. High-resolution, high-contrast second harmonic generation (SHG) imaging of corneal tissue based on the intrinsic structure of collagen is discussed. The three-dimensional corneal ultrastructure in depths up to hundreds of microns can be probed noninvasively, without any staining or mechanical slicing. As an important application of second harmonic imaging in ophthalmology, the modification of corneal ultrastructure using femtosecond laser intrastromal ablation is systematically investigated to evaluate next-generation refractive surgical approaches.