Deutsch
 
Hilfe Datenschutzhinweis Impressum
  DetailsucheBrowse

Datensatz

DATENSATZ AKTIONENEXPORT
Zur Ablage hinzufügen
  Lokale TagsFreigabegeschichteDetailsÜbersicht

Freigegeben
Zeitschriftenartikel

A genome-wide resource for the analysis of protein localisation in Drosophila

MPG-Autoren
/persons/resource/persons145330

Barz,  Christiane
Schnorrer, Frank / Muscle Dynamics, Max Planck Institute of Biochemistry, Max Planck Society;

/persons/resource/persons78085

Hein,  Marco Y.
Mann, Matthias / Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Max Planck Society;

/persons/resource/persons192006

Stender,  Bettina
Schnorrer, Frank / Muscle Dynamics, Max Planck Institute of Biochemistry, Max Planck Society;

/persons/resource/persons79377

Ferreira,  Irene R. S.
Schnorrer, Frank / Muscle Dynamics, Max Planck Institute of Biochemistry, Max Planck Society;

/persons/resource/persons181439

Finkl,  Katja
Schnorrer, Frank / Muscle Dynamics, Max Planck Institute of Biochemistry, Max Planck Society;

/persons/resource/persons192011

Plewka,  Nicole
Schnorrer, Frank / Muscle Dynamics, Max Planck Institute of Biochemistry, Max Planck Society;

/persons/resource/persons78356

Mann,  Matthias
Mann, Matthias / Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Max Planck Society;

/persons/resource/persons78656

Schnorrer,  Frank
Schnorrer, Frank / Muscle Dynamics, Max Planck Institute of Biochemistry, Max Planck Society;

Externe Ressourcen
Es sind keine externen Ressourcen hinterlegt
Volltexte (beschränkter Zugriff)
Für Ihren IP-Bereich sind aktuell keine Volltexte freigegeben.
Volltexte (frei zugänglich)

elife-12068-v2.pdf
(beliebiger Volltext), 10MB

Ergänzendes Material (frei zugänglich)
Es sind keine frei zugänglichen Ergänzenden Materialien verfügbar
Zitation

Sarov, M., Barz, C., Jambor, H., Hein, M. Y., Schmied, C., Suchold, D., et al. (2016). A genome-wide resource for the analysis of protein localisation in Drosophila. eLife, 5: e12068. doi:10.7554/eLife.12068.


Zitierlink: https://hdl.handle.net/11858/00-001M-0000-002A-1991-E
Zusammenfassung
The Drosophila genome contains >13000 protein-coding genes, the majority of which remain poorly investigated. Important reasons include the lack of antibodies or reporter constructs to visualise these proteins. Here, we present a genome-wide fosmid library of 10000 GFP-tagged clones, comprising tagged genes and most of their regulatory information. For 880 tagged proteins, we created transgenic lines, and for a total of 207 lines, we assessed protein expression and localisation in ovaries, embryos, pupae or adults by stainings and live imaging approaches. Importantly, we visualised many proteins at endogenous expression levels and found a large fraction of them localising to subcellular compartments. By applying genetic complementation tests, we estimate that about two-thirds of the tagged proteins are functional. Moreover, these tagged proteins enable interaction proteomics from developing pupae and adult flies. Taken together, this resource will boost systematic analysis of protein expression and localisation in various cellular and developmental contexts.