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A genome-wide resource for the analysis of protein localisation in Drosophila

MPS-Authors
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Barz,  Christiane
Schnorrer, Frank / Muscle Dynamics, Max Planck Institute of Biochemistry, Max Planck Society;

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Hein,  Marco Y.
Mann, Matthias / Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Max Planck Society;

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Stender,  Bettina
Schnorrer, Frank / Muscle Dynamics, Max Planck Institute of Biochemistry, Max Planck Society;

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Ferreira,  Irene R. S.
Schnorrer, Frank / Muscle Dynamics, Max Planck Institute of Biochemistry, Max Planck Society;

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Finkl,  Katja
Schnorrer, Frank / Muscle Dynamics, Max Planck Institute of Biochemistry, Max Planck Society;

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Plewka,  Nicole
Schnorrer, Frank / Muscle Dynamics, Max Planck Institute of Biochemistry, Max Planck Society;

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Mann,  Matthias
Mann, Matthias / Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Max Planck Society;

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Schnorrer,  Frank
Schnorrer, Frank / Muscle Dynamics, Max Planck Institute of Biochemistry, Max Planck Society;

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Citation

Sarov, M., Barz, C., Jambor, H., Hein, M. Y., Schmied, C., Suchold, D., et al. (2016). A genome-wide resource for the analysis of protein localisation in Drosophila. eLife, 5: e12068. doi:10.7554/eLife.12068.


Cite as: http://hdl.handle.net/11858/00-001M-0000-002A-1991-E
Abstract
The Drosophila genome contains >13000 protein-coding genes, the majority of which remain poorly investigated. Important reasons include the lack of antibodies or reporter constructs to visualise these proteins. Here, we present a genome-wide fosmid library of 10000 GFP-tagged clones, comprising tagged genes and most of their regulatory information. For 880 tagged proteins, we created transgenic lines, and for a total of 207 lines, we assessed protein expression and localisation in ovaries, embryos, pupae or adults by stainings and live imaging approaches. Importantly, we visualised many proteins at endogenous expression levels and found a large fraction of them localising to subcellular compartments. By applying genetic complementation tests, we estimate that about two-thirds of the tagged proteins are functional. Moreover, these tagged proteins enable interaction proteomics from developing pupae and adult flies. Taken together, this resource will boost systematic analysis of protein expression and localisation in various cellular and developmental contexts.