English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT

Released

Journal Article

A three-step MTOC fragmentation mechanism facilitates bipolar spindle assembly in mouse oocytes.

MPS-Authors
/persons/resource/persons188399

Schuh,  M.
Department of Meiosis, MPI for Biophysical Chemistry, Max Planck Society;

Locator
Fulltext (public)

2262032.pdf
(Publisher version), 3MB

Supplementary Material (public)

2262032_Suppl_1.pdf
(Supplementary material), 2MB

2262032_Suppl_2.mov
(Supplementary material), 676KB

2262032_Suppl_3.mov
(Supplementary material), 575KB

2262032_Suppl_4.mov
(Supplementary material), 225KB

2262032_Suppl_5.mov
(Supplementary material), 41KB

2262032_Suppl_6.mov
(Supplementary material), 2MB

2262032_Suppl_7.mov
(Supplementary material), 929KB

2262032_Suppl_8.mov
(Supplementary material), 427KB

Citation

Clift, D., & Schuh, M. (2015). A three-step MTOC fragmentation mechanism facilitates bipolar spindle assembly in mouse oocytes. Nature Communications, 6: 7217. doi:10.1038/ncomms8217.


Cite as: http://hdl.handle.net/11858/00-001M-0000-002A-1B59-D
Abstract
Assembly of a bipolar microtubule spindle is essential for accurate chromosome segregation. In somatic cells, spindle bipolarity is determined by the presence of exactly two centrosomes. Remarkably, mammalian oocytes do not contain canonical centrosomes. This study reveals that mouse oocytes assemble a bipolar spindle by fragmenting multiple acentriolar microtubule-organizing centres (MTOCs) into a high number of small MTOCs to be able to then regroup and merge them into two equal spindle poles. We show that MTOCs are fragmented in a three-step process. First, PLK1 triggers a decondensation of the MTOC structure. Second, BicD2-anchored dynein stretches the MTOCs into fragmented ribbons along the nuclear envelope. Third, KIF11 further fragments the MTOCs following nuclear envelope breakdown so that they can be evenly distributed towards the two spindle poles. Failure to fragment MTOCs leads to defects in spindle assembly, which delay chromosome individualization and congression, putting the oocyte at risk of aneuploidy.