English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT

Released

Journal Article

Error-prone chromosome-mediated spindle assembly favors chromosome segregation defects in human oocytes.

MPS-Authors
/persons/resource/persons188399

Schuh,  M.
Department of Meiosis, MPI for Biophysical Chemistry, Max Planck Society;

External Ressource
Fulltext (public)
There are no public fulltexts stored in PuRe
Supplementary Material (public)

2262044_Suppl_1.pdf
(Supplementary material), 1012KB

2262044_Suppl_2.mov
(Supplementary material), 6MB

2262044_Suppl_3.mov
(Supplementary material), 4MB

2262044_Suppl_4.mov
(Supplementary material), 4MB

2262044_Suppl_5.mov
(Supplementary material), 10MB

2262044_Suppl_6.mov
(Supplementary material), 3MB

2262044_Suppl_7.mov
(Supplementary material), 9MB

Citation

Holubcová, Z., Blayney, M., Elder, K., & Schuh, M. (2015). Error-prone chromosome-mediated spindle assembly favors chromosome segregation defects in human oocytes. Science, 348(6239), 1143-1147. doi:10.1126/science.aaa9529.


Cite as: http://hdl.handle.net/11858/00-001M-0000-002A-1B71-6
Abstract
Aneuploidy in human eggs is the leading cause of pregnancy loss and several genetic disorders such as Down syndrome. Most aneuploidy results from chromosome segregation errors during the meiotic divisions of an oocyte, the egg’s progenitor cell. The basis for particularly error-prone chromosome segregation in human oocytes is not known. We analyzed meiosis in more than 100 live human oocytes and identified an error-prone chromosome-mediated spindle assembly mechanism as a major contributor to chromosome segregation defects. Human oocytes assembled a meiotic spindle independently of either centrosomes or other microtubule organizing centers. Instead, spindle assembly was mediated by chromosomes and the small guanosine triphosphatase Ran in a process requiring ~16 hours. This unusually long spindle assembly period was marked by intrinsic spindle instability and abnormal kinetochore-microtubule attachments, which favor chromosome segregation errors and provide a possible explanation for high rates of aneuploidy in human eggs.