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Journal Article

Anticancer agent 3-bromopyruvic acid forms a conjugate with glutathione.

MPS-Authors
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Jaremko,  L.
Research Group of Protein Structure Determination using NMR, MPI for biophysical chemistry, Max Planck Society;

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Jaremko,  M.
Research Group of Protein Structure Determination using NMR, MPI for biophysical chemistry, Max Planck Society;

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Citation

Sadowska-Bartosz, I., Szewczyk, R., Jaremko, L., Jaremko, M., & Bartosz, G. (2016). Anticancer agent 3-bromopyruvic acid forms a conjugate with glutathione. Pharmacological Reports, 68(2), 502-505. doi:10.1016/j.pharep.2015.11.007.


Cite as: http://hdl.handle.net/11858/00-001M-0000-002A-1C0D-2
Abstract
Background: 3-Bromopyruvic acid (3-BP), a glycolytic inhibitor and a promising anticancer compound, induces oxidative stress and depletes cells of glutathione (GSH). The causes of GSH loss remain unclear. The aim of this study was to ascertain whether 3-BP forms a conjugate with glutathione. Methods: GSH was incubated with various amounts of 3-BP and the extent of reaction was titrated with H-1 NMR and H-1-H-1 NMR. The reaction outcome was identified by MS/MS. Intracellular formation of the conjugate was assessed in cells treated with 3-BP and 3-BP(C-13) and analyzed using the targeted LC-MS/MS method in negative ionization MRM mode. Results: 3-BP was found to react with GSH in a 1:1 ratio forming an S-conjugate. The same conjugate was formed intracellularly in erythrocytes and MCF-7 cells. Conclusions: 3-BP reacts with GSH in the absence of cells and intracellularly. This reaction appears to be the main cause of GSH loss in 3-BP treated cells.