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Functional fluorescent Ca2+ indicator proteins in transgenic mice under TET control.

MPS-Authors
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Hasan,  Mazahir T.
Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society;
Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society;

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Friedrich,  Rainer W.
Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society;

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Euler,  Thomas
Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society;

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Larkum,  Matthew E.
Department of Cell Physiology, Max Planck Institute for Medical Research, Max Planck Society;

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Giese,  Günter
Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society;

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Both,  Matthias
Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society;

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Dübel,  Jens
Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society;

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Waters,  David Jack
Department of Cell Physiology, Max Planck Institute for Medical Research, Max Planck Society;

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Denk,  Winfried
Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society;

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Citation

Hasan, M. T., Friedrich, R. W., Euler, T., Larkum, M. E., Giese, G., Both, M., et al. (2004). Functional fluorescent Ca2+ indicator proteins in transgenic mice under TET control. PLoS Biology, 2(6): e163, pp. 763-775. doi:10.1371/journal.pbio.0020163.


Cite as: http://hdl.handle.net/11858/00-001M-0000-002A-26D8-7
Abstract
Genetically encoded fluorescent calcium indicator proteins (FCIPs) are promising tools to study calcium dynamics in many activity-dependent molecular and cellular processes. Great hopes-for the measurement of population activity, in particular-have therefore been placed on calcium indicators derived from the green fluorescent protein and their expression in (selected) neuronal populations. Calcium transients can rise within milliseconds, making them suitable as reporters of fast neuronal activity. We here report the production of stable transgenic mouse lines with two different functional calcium indicators, inverse pericam and camgaroo-2, under the control of the tetracycline-inducible promoter. Using a variety of in vitro and in vivo assays, we find that stimuli known to increase intracellular calcium concentration (somatically triggered action potentials (APs) and synaptic and sensory stimulation) can cause substantial and rapid changes in FCIP fluorescence of inverse pericam and camgaroo-2.