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Journal Article

Single-Stranded Tile Stoppers for Interlocked DNA Architectures


Famulok,  Michael
Max Planck Fellow Chemical Biology, Center of Advanced European Studies and Research (caesar), Max Planck Society;

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Valero, J., Lohmann, F., Keppner, D., & Famulok, M. (2016). Single-Stranded Tile Stoppers for Interlocked DNA Architectures. ChemBioChem: A European Journal of Chemical Biology, 17, 1146-1149. doi:10.1002/cbic.201500685.

Cite as: http://hdl.handle.net/11858/00-001M-0000-002A-29A7-C
Interlocked DNA architectures are useful for DNA nanotechnology because of their mechanically bonded components, which can move relative to one another without disassembling. We describe the design, synthesis, and characterization of novel single‐stranded tile (SST) stoppers for the assembly of interlocked DNA architectures. SST stoppers are shown to self‐assemble into a square‐shaped rigid structure upon mixing 97 oligodeoxynucleotide (ODN) strands. The structures are equipped with a sticky end that is designed for hybridization with the sticky ends of a dsDNA axle of a DNA rotaxane. Because the diameter of the macrocycle threaded onto the axle is 14 nm, the dimension of the square‐shaped stopper was designed to be bulky enough to prevent the dethreading of the macrocycle. An asymmetric rotaxane with a SST‐ and a ring‐shaped stopper featuring two stations for hybridization of the macrocycle to the axle was assembled. The macrocycle can be directed towards one or the other station upon triggering with fuel ODNs.