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High refractive index immersion liquid for superresolution 3D imaging using sapphire-based aplanatic numerical aperture increasing lens optics.

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Laskar,  Junaid M.
Group Statistical mechanics of granular media, Department of Dynamics of Complex Fluids, Max Planck Institute for Dynamics and Self-Organization, Max Planck Society;

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Herminghaus,  S.
Group Granular matter and irreversibility, Department of Dynamics of Complex Fluids, Max Planck Institute for Dynamics and Self-Organization, Max Planck Society;

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Schröter,  Matthias
Group Statistical mechanics of granular media, Department of Dynamics of Complex Fluids, Max Planck Institute for Dynamics and Self-Organization, Max Planck Society;

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Citation

Laskar, J. M., Kumar, P. S., Herminghaus, S., Daniels, K. E., & Schröter, M. (2016). High refractive index immersion liquid for superresolution 3D imaging using sapphire-based aplanatic numerical aperture increasing lens optics. Applied Optics, 55(12), 3165-3169. doi:10.1364/AO.55.003165.


Cite as: http://hdl.handle.net/11858/00-001M-0000-002A-2B3A-3
Abstract
Optically transparent immersion liquids with refractive index (n ∼ 1.77) to match the sapphire-based aplanatic numerical aperture increasing lens (aNAIL) are necessary for achieving deep 3D imaging with high spatial resolution. We report that antimony tribromide (SbBr3) salt dissolved in liquid diiodomethane (CH2I2) provides a new high refractive index immersion liquid for optics applications. The refractive index is tunable from n 1.74 (pure) to n 1.873 (saturated), by adjusting either salt concentration or temperature; this allows it to match (or even exceed) the refractive index of sapphire. Importantly, the solution gives excellent light transmittance in the ultraviolet to near-infrared range, an improvement over commercially available immersion liquids. This refractive-index-matched immersion liquid formulation has enabled us to develop a sapphire-based aNAIL objective that has both high numerical aperture (NA 1.17) and long working distance (WD 12 mm). This opens up new possibilities for deep 3D imaging with high spatial resolution.