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Parallelized ultra-high throughput microfluidic emulsifier for multiplex kinetic assays

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Lim,  Jiseok
Max Planck Research Group Droplets, Membranes and Interfaces, Max Planck Institute for Dynamics and Self-Organization, Max Planck Society;

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Baret,  Jean-Christophe
Max Planck Research Group Droplets, Membranes and Interfaces, Max Planck Institute for Dynamics and Self-Organization, Max Planck Society;

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Citation

Lim, J., Caen, O., Vrignon, J., Konrad, M., Taly, V., & Baret, J.-C. (2015). Parallelized ultra-high throughput microfluidic emulsifier for multiplex kinetic assays. Biomicrofluidics, 9(3): 034101. doi:10.1063/1.4919415.


Cite as: http://hdl.handle.net/11858/00-001M-0000-002A-3997-F
Abstract
Droplet-based microfluidic technologies are powerful tools for applications requiring high-throughput, for example, in biochemistry or material sciences. Several systems have been proposed for the high-throughput production of monodisperse emulsions by parallelizing multiple droplet makers. However, these systems have two main limitations: (1) they allow the use of only a single disperse phase; (2) they are based on multiple layer microfabrication techniques. We present here a pipette-and-play solution offering the possibility of manipulating simultaneously 10 different disperse phases on a single layer device. This system allows high-throughput emulsion production using aqueous flow rates of up to 26 ml/h (>110 000 drops/s) leading to emulsions with user-defined complex chemical composition. We demonstrate the multiplex capabilities of our system by measuring the kinetics of β-galactosidase in droplets using nine different concentrations of a fluorogenic substrate.