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Journal Article

Quantitative analysis of the Escherichia coli proteome.

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Wisniewski,  Jacek R.
Mann, Matthias / Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Max Planck Society;

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Citation

Wisniewski, J. R., & Rakus, D. (2014). Quantitative analysis of the Escherichia coli proteome. Data in brief, 1, 7-11. doi:10.1016/j.dib.2014.08.004.


Cite as: https://hdl.handle.net/11858/00-001M-0000-002A-3AD0-7
Abstract
Escherichia coli (strain ATCC 25922 in a stationary culture) cells were lysed with SDS and the lysates were processed according MED-FASP protocol. The released peptides were analyzed by LC-MS/MS. Protein content per bacterial cell was calculated on the basis of the DNA content. Absolute protein quantitation was performed using the 'Total Protein Approach'. The data are supplied in the article.