Two-photon excited autofluorescence imaging of human retinal pigment epithelial 
cells

Han, Meng; Bindewald-Wittich, Almut; Holz, Frank G.; Giese, Günter; Niemz, Markolf H.; Snyder, Sarah Rebecca; Sun, Hui; Yu, Jiayi; Agopov, Mikael; La Schiazza, Olivier; Bille, Josef F.

doi:10.1117/1.2171649

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Two-photon excited autofluorescence imaging of human retinal pigment epithelial cells

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Giese, Günter
Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society;

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Snyder, Sarah Rebecca
Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society;

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Agopov, Mikael
Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society;

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La Schiazza, Olivier
Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society;

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https://dx.doi.org/10.1117/1.2171649
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http://biomedicaloptics.spiedigitallibrary.org/data/Journals/BIOMEDO/22407/010501_1.pdf
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Citation

Han, M., Bindewald-Wittich, A., Holz, F. G., Giese, G., Niemz, M. H., Snyder, S. R., et al. (2006). Two-photon excited autofluorescence imaging of human retinal pigment epithelial cells. Journal of Biomedical Optics, 11(1), 10501-10501. doi:10.1117/1.2171649.

Cite as: https://hdl.handle.net/11858/00-001M-0000-002A-E8EE-9

Abstract

Degeneration of retinal pigment epithelial (RPE) cells severely impairs the visual function of retina photoreceptors. However, little is known about the events that trigger the death of RPE cells at the subcellular level. Two-photon excited autofluorescence (TPEF) imaging of RPE cells proves to be well suited to investigate both the morphological and the spectral characteristics of the human RPE cells. The dominant fluorophores of autofluorescence derive from lipofuscin (LF) granules that accumulate in the cytoplasm of the RPE cells with increasing age. Spectral TPEF imaging reveals the existence of abnormal LF granules with blue shifted autofluorescence in RPE cells of aging patients and brings new insights into the complicated composition of the LF granules. Based on a proposed two-photon laser scanning ophthalmoscope, TPEF imaging of the living retina may be valuable for diagnostic and pathological studies of age related eye diseases.