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Journal Article

Reconstruction of firing rate changes across neuronal populations by temporally deconvolved Ca2+ imaging

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Yaksi,  Emre
Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society;

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Friedrich,  Rainer W.
Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society;

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Citation

Yaksi, E., & Friedrich, R. W. (2006). Reconstruction of firing rate changes across neuronal populations by temporally deconvolved Ca2+ imaging. Nature methods, 3(5), 377-383. doi:10.1038/nmeth874.


Cite as: http://hdl.handle.net/11858/00-001M-0000-002A-EE57-7
Abstract
Methods to record action potential (AP) firing in many individual neurons are essential to unravel the function of complex neuronal circuits in the brain. A promising approach is bolus loading of Ca(2+) indicators combined with multiphoton microscopy. Currently, however, this technique lacks cell-type specificity, has low temporal resolution and cannot resolve complex temporal firing patterns. Here we present simple solutions to these problems. We identified neuron types by colocalizing Ca(2+) signals of a red-fluorescing indicator with genetically encoded markers. We reconstructed firing rate changes from Ca(2+) signals by temporal deconvolution. This technique is efficient, dramatically enhances temporal resolution, facilitates data interpretation and permits analysis of odor-response patterns across thousands of neurons in the zebrafish olfactory bulb. Hence, temporally deconvolved Ca(2+) imaging (TDCa imaging) resolves limitations of current optical recording techniques and is likely to be widely applicable because of its simplicity, robustness and generic principle.