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Journal Article

LOVTRAP: an optogenetic system for photoinduced protein dissociation

MPS-Authors
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Winkler,  Andreas
Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society;

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Tarnawski,  Miroslaw
Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society;

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Schlichting,  Ilme
Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society;

External Resource

http://www.nature.com/nmeth/journal/vaop/ncurrent/pdf/nmeth.3926.pdf
(Any fulltext)

https://dx.doi.org/10.1038/nmeth.3926
(Any fulltext)

http://www.nature.com/nmeth/journal/vaop/ncurrent/extref/nmeth.3926-S1.pdf
(Supplementary material)

http://www.nature.com/nmeth/journal/vaop/ncurrent/extref/nmeth.3926-S2.zip
(Supplementary material)

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Citation

Wang, H., Vilela, M., Winkler, A., Tarnawski, M., Schlichting, I., Yumerefendi, H., et al. (2016). LOVTRAP: an optogenetic system for photoinduced protein dissociation. Nature methods, 13(9), 755-758. doi:10.1038/nmeth.3926.


Cite as: https://hdl.handle.net/11858/00-001M-0000-002B-1FDA-D
Abstract
LOVTRAP is an optogenetic approach for reversible light-induced protein dissociation using protein A fragments that bind to the LOV domain only in the dark, with tunable kinetics and a >150-fold change in the dissociation constant (Kd). By reversibly sequestering proteins at mitochondria, we precisely modulated the proteins' access to the cell edge, demonstrating a naturally occurring 3-mHz cell-edge oscillation driven by interactions of Vav2, Rac1, and PI3K proteins.