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Local pH gradient initiated by light on TiO2 for light-triggered modulation of polyhistidine-tagged proteins

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Baidukova,  Olga
Katja Skorb (Indep. Res.), Biomaterialien, Max Planck Institute of Colloids and Interfaces, Max Planck Society;

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Skorb,  Ekaterina. V.
Katja Skorb (Indep. Res.), Biomaterialien, Max Planck Institute of Colloids and Interfaces, Max Planck Society;

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Citation

Andreeva, D. V., Melnyk, I., Baidukova, O., & Skorb, E. V. (2016). Local pH gradient initiated by light on TiO2 for light-triggered modulation of polyhistidine-tagged proteins. ChemElectroChem, 3(9), 1306-1310. doi:10.1002/celc.201600268.


Cite as: http://hdl.handle.net/11858/00-001M-0000-002B-29FD-E
Abstract
A new principle of photo-assisted spatial desorption of (poly)histidine-tagged (His-Tag) proteins on a TiO2 surface is suggested. A semiconductor TiO2 surface is decorated by layer-by-layer (LbL) assembly of a strong polyelectrolyte, namely, polystyrene sulfonate (PSS), and nickel–nitrilotriacetic acid (NTA). The PSS/NTA multilayer architecture provides n-fold (n×NTA) binding efficiency for more precise protein recognition in comparison to existing molecular His-Tag protein recognition with one- and threefold multiplication (1×NTA, 3×NTA). Spatially resolved desorption of proteins is regulated by non- photodestructive short-term low-intensity light irradiation (<5 mW cm−2). The local pH shift on irradiated TiO2 selectively affects the pH-sensitive NTA/protein complex, but not the LbL assembly of PSS and NTA, which is stable in a broad pH range.