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Characterization of 36 polymorphic microsatellite loci in the Kentish plover (Charadrius alexandrinus) including two sex-linked loci and their amplification in four other Charadrius species

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Küpper, C., Horsburgh, G. J., Dawson, D. A., Ffrench-Constant, R., Székely, T., & Burke, T. (2007). Characterization of 36 polymorphic microsatellite loci in the Kentish plover (Charadrius alexandrinus) including two sex-linked loci and their amplification in four other Charadrius species. Molecular Ecology Notes, 7(1), 35-39. doi:10.1111/j.1471-8286.2006.01517.x.


Cite as: http://hdl.handle.net/11858/00-001M-0000-002B-2550-0
Abstract
We isolated 45 new Kentish plover (Charadrius alexandrinus) microsatellite loci. These were tested for polymorphism in 42 Kentish plovers breeding in the Cukurova Delta, Turkey. Thirty-six of the 45 loci were polymorphic with observed heterozygosity varying between 0.22 and 0.93. Genotypes of individuals of known sex indicated that two loci were sex-linked (Calex-26 is located on the Z chromosome and Calex-31 on the W chromosome). Additionally, we tested all loci for amplification in four other species of Charadridae (Kittlitz's plover, Madagascar plover, three-banded plover and white-fronted plover). On average 34 loci amplified per species (range 29-36).