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Journal Article

Easily prepared holey films for use in cryo-electron microscopy

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Jahn,  Werner
Emeritus Group Biophysics, Max Planck Institute for Medical Research, Max Planck Society;

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Citation

Jahn, W. (1995). Easily prepared holey films for use in cryo-electron microscopy. Journal of Microscopy, 179(3), 333-334. doi:10.1111/j.1365-2818.1995.tb03649.x.


Cite as: http://hdl.handle.net/11858/00-001M-0000-002B-49F8-1
Abstract
Holey films or nets may be produced from an emulsion of glycerol/water in a solution of a mixture of Formvar® and Triafol® in dichloromethane, spread on mica. After being floated off the mica support the films may be treated with ethyl acetate to transform 'pseudoholes' into holes or nets.