English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
Add to Basket
  Local TagsRelease HistoryDetailsSummary

Released
Journal Article

Mechanism of the activation of proteinase inhibitor synthesis by systemin involves β-sheet structure, a specific DNA-binding protein domain

MPS-Authors
/persons/resource/persons198721

Slósarek,  Genowefa
Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons93660

Kalbitzer,  Hans Robert
Emeritus Group Biophysics, Max Planck Institute for Medical Research, Max Planck Society;

External Resource

http://www.sciencedirect.com/science/article/pii/S104784778571026X/pdf?md5=b6e6e77be719406bef5a0132bc36c19c&pid=1-s2.0-S104784778571026X-main.pdf
(Any fulltext)

http://dx.doi.org/10.1006/jsbi.1995.1026
(Any fulltext)

Fulltext (restricted access)
There are currently no full texts shared for your IP range.
Fulltext (public)
There are no public fulltexts stored in PuRe
Supplementary Material (public)
There is no public supplementary material available
Citation

Slósarek, G., Kalbitzer, H. R., Mucha, P., Rekowski, P., Kupryszewski, G., Giel-Pietraszuk, M., et al. (1995). Mechanism of the activation of proteinase inhibitor synthesis by systemin involves β-sheet structure, a specific DNA-binding protein domain. Journal of Structural Biology, 115(1), 30-36. doi:10.1006/jsbi.1995.1026.


Cite as: https://hdl.handle.net/11858/00-001M-0000-002B-4FE0-1
Abstract
We analyzed a tertiary structure of systemin, the first identified polypeptide plant hormone, using two-dimensional NMR spectroscopy. From these data and molecular dynamics calculations we concluded that the peptide can adopt a Z-like-β-sheet structure, which has previously been found in many specific DNA-binding proteins. Using DNA-cellulose affinity chromatography, we showed that systemin binds strongly to DNA. We suggest that the specific systemin-DNA interaction, particularly in a promoter region of the proteinase inhibitors, could effect gone expression and thus explain the biological activity of systemin.