A look at membrane patches with a scanning force microscope

Hörber, J. K. Heinrich; Mosbacher, Johannes; Häberle, Walter; Ruppersberg, J. Peter; Sakmann, Bert

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A look at membrane patches with a scanning force microscope

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Mosbacher, Johannes
Department of Cell Physiology, Max Planck Institute for Medical Research, Max Planck Society;

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Ruppersberg, J. Peter
Department of Cell Physiology, Max Planck Institute for Medical Research, Max Planck Society;

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Sakmann, Bert
Department of Cell Physiology, Max Planck Institute for Medical Research, Max Planck Society;

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http://www.sciencedirect.com/science/article/pii/S0006349595803462/pdf?md5=dce26ff47c8f5a1ee208d17b9784e753&pid=1-s2.0-S0006349595803462-main.pdf
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http://dx.doi.org/10.1016/S0006-3495(95)80346-2
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Citation

Hörber, J. K. H., Mosbacher, J., Häberle, W., Ruppersberg, J. P., & Sakmann, B. (1995). A look at membrane patches with a scanning force microscope. Biophysical Journal, 68(5), 1687-1693. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/7612812.

Cite as: https://hdl.handle.net/11858/00-001M-0000-002B-5658-4

Abstract

We combined scanning force microscopy with patch-clamp techniques in the same experimental setup and obtained images of excised membrane patches spanning the tip of a glass pipette. These images indicate that cytoskeleton structures are still present in such membrane patches and form a strong connection between the membrane and the glass wall. This gives the membrane patch the appearance of a tent, stabilized by a scaffold of ropes. The lateral resolution of the images depends strongly on the observed structures and can reach values as low as 10 nm on the cytoskeleton elements of a (inside-out) patch. The observations suggest that measurements of membrane elasticity can be made, opening the way for further studies on mechanical properties of cell membranes.