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Evaluating atomic models of F-actin with an undecagold-tagged phalloidin derivative

MPG-Autoren
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Jahn,  Werner
Emeritus Group Biophysics, Max Planck Institute for Medical Research, Max Planck Society;

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Faulstich,  Heinz
Department of Molecular Cell Research, Max Planck Institute for Medical Research, Max Planck Society;

Externe Ressourcen

http://www.sciencedirect.com/science/article/pii/S0022283697915291/pdfft?md5=e9e30a5222dd1653607191e948e89a73&pid=1-s2.0-S0022283697915291-main.pdf
(beliebiger Volltext)

http://dx.doi.org/10.1006/jmbi.1997.1529
(beliebiger Volltext)

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Zitation

Steinmetz, M. O., Stoffler, D., Müller, S. A., Jahn, W., Wolpensinger, B., Goldie, K. N., et al. (1998). Evaluating atomic models of F-actin with an undecagold-tagged phalloidin derivative. Journal of Molecular Biology (London), 276(1), 1-6. doi:10.1006/jmbi.1997.1529.


Zitierlink: https://hdl.handle.net/11858/00-001M-0000-002B-78F8-6
Zusammenfassung
We have prepared an undecagold-tagged phalloidin derivative to determine this mushroom toxin's binding site and orientation within the F-actin filament by scanning transmission electron microscopy (STEM) and 3-D helical reconstruction. Remarkably, when stoichiometrically bound to F-actin, the undecagold moiety of the derivative could be directly visualized by STEM along the two half-staggered long-pitch helical strands of single filaments. Most importantly, the structural data obtained when combined with various biochemical constraints enabled us to critically evaluate two distinct atomic models of the F-actin filament (i.e. the Holmes-Lorenz versus the Schutt-Lindberg model). Taken together, our data are in excellent agreement with the Holmes-Lorenz model.