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Detection of protein complex interactions via a Blue Native-PAGE retardation assay

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Swamy,  Mahima
Research Group and Chair of Molecular Immunology of the University of Freiburg, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society;

Molnar,  Eszter
Max Planck Society;

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Bock,  Thomas
Research Group and Chair of Molecular Immunology of the University of Freiburg, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society;

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Wollscheid,  Bernd
Research Group and Chair of Molecular Immunology of the University of Freiburg, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society;

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Schamel,  Wolfgang W. A.
Research Group and Chair of Molecular Immunology of the University of Freiburg, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society;

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Citation

Swamy, M., Molnar, E., Bock, T., Bausch-Fluck, D., Wollscheid, B., & Schamel, W. W. A. (2009). Detection of protein complex interactions via a Blue Native-PAGE retardation assay. Analytical Biochemstry, 392, 177-179.


Cite as: https://hdl.handle.net/11858/00-001M-0000-002B-8FA8-7
Abstract
We describe the Blue Native (BN)-PAGE retardation assay for the detection of interactions of biomolecules with protein complexes. Potential interactors of proteins are included in the BN gel matrix, resulting in retardation of proteins that interact with the added molecule. After validation using the T-cell antigen receptor, we applied the assay for a general identification of dextran interactors in combination with mass spectroscopy. The proteomic screen revealed triosephosphate isomerase oligomer as a dextranbinding, high MR complex.