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Journal Article

Components of the ligand for a Ni++ reactive human T cell clone

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/persons/resource/persons191364

Vollmer,  Jörg
Emeritus Group: Cellular Immunology, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society;

/persons/resource/persons191229

Moulon,  Corinne
Emeritus Group: Cellular Immunology, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society;

/persons/resource/persons191380

Weltzien,  Hans-Ulrich
Emeritus Group: Cellular Immunology, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society;

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Citation

Lu, L., Vollmer, J., Moulon, C., Weltzien, H.-U., Marrack, P., & Kappler, J. (2003). Components of the ligand for a Ni++ reactive human T cell clone. Journal of Experimental Medicine, 197(5), 567-574.


Cite as: http://hdl.handle.net/11858/00-001M-0000-002B-9555-E
Abstract
The major histocompatibility complex (MHC) restriction element for a human Ni2+ reactive T cell, ANi-2.3, was identified as DR52c. A series of experiments established that the functional ligand for this T cell was a preformed complex of Ni2+ bound to the combination of DR52c and a specific peptide that was generated in human and mouse B cells, but not in fibroblasts nor other antigen processing-deficient cells. In addition, ANi-2.3 recognition of this complex was dependent on βHis81 of the MHC β chain, suggesting a role for this amino acid in Ni2+ binding to MHC. We propose a general model for Ni2+ recognition in which β His81 and two amino acids from the NH2-terminal part of the MHC bound peptide coordinate Ni2+ which then interacts with some portion of the Vα CDR1 or CDR2 region.