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Journal Article

FBXO3 protein promotes ubiquitylation and transcriptional activity of AIRE (Autoimmune Regulator).

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Zumer,  K.
Department of Molecular Biology, MPI for Biophysical Chemistry, Max Planck Society;

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Citation

Shao, W., Zumer, K., Fujinaga, K., & Peterlin, B. M. (2016). FBXO3 protein promotes ubiquitylation and transcriptional activity of AIRE (Autoimmune Regulator). Journal of Biological Chemistry, 291(34), 17953-17963. doi:10.1074/jbc.M116.724401.


Cite as: http://hdl.handle.net/11858/00-001M-0000-002B-834A-A
Abstract
The autoimmune regulator (AIRE) is a transcription factor which is expressed in medullary thymic epithelial cells. It directs the expression of otherwise tissue-specific antigens, which leads to the elimination of autoreactive T cells during development. AIRE is modified post-translationally by phosphorylation and ubiquitylation. In this report we connected these modifications. AIRE, which is phosphorylated on two specific residues near its N terminus, then binds to the F-box protein 3 (FBXO3) E3 ubiquitin ligase. In turn, this SCFFBXO3 (SKP1-CUL1-F box) complex ubiquitylates AIRE, increases its binding to the positive transcription elongation factor b (P-TEFb), and potentiates its transcriptional activity. Because P-TEFb is required for the transition from initiation to elongation of transcription, this interaction ensures proper expression of AIRE-responsive tissue-specific antigens in the thymus.