Perforated support foils with pre-defined hole size, shape and arrangement

Tichelaar, Willem; Ermantraut, E.; Wohlfart, K.

doi:10.1016/S0304-3991(98)00025-4

Item

ITEM ACTIONSEXPORT

Add to Basket

Local TagsRelease HistoryDetailsSummary

Released

Journal Article

Perforated support foils with pre-defined hole size, shape and arrangement

MPS-Authors

/persons/resource/persons95648

Tichelaar, Willem
Department of Cell Physiology, Max Planck Institute for Medical Research, Max Planck Society;

External Resource

http://www.sciencedirect.com/science/article/pii/S0304399198000254/pdfft?md5=367b3c585e8ae11c85e24d780f8766f5&pid=1-s2.0-S0304399198000254-main.pdf
(Any fulltext)

http://dx.doi.org/10.1016/S0304-3991(98)00025-4
(Any fulltext)

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

Fulltext (public)

There are no public fulltexts stored in PuRe

Supplementary Material (public)

There is no public supplementary material available

Citation

Tichelaar, W., Ermantraut, E., & Wohlfart, K. (1998). Perforated support foils with pre-defined hole size, shape and arrangement. Ultramicroscopy, 74(1-2), 75-81. doi:10.1016/S0304-3991(98)00025-4.

Cite as: https://hdl.handle.net/11858/00-001M-0000-002B-9B89-E

Abstract

Perforated support foils with holes of pre-defined size, shape and arrangement and with hole sizes down to the sub-micrometer range, named Quantifoil, are presented. The foils are fabricated using semiconductor lithographic techniques. For the first time, a sacrificial layer that consists of a biopolymer, i.e. glutaraldehyde cross-linked gelatin, is used. This layer is removed by proteinase treatment, thus introducing an enzymatic reaction as a tool in microsystems technology. The foils are particularly beneficial in electron microscopy, when a specimen support is required with holes smaller than those attainable with metal grids (∼10 μm). Their advantages in cryo-electron microscopy, for example, are illustrated. Furthermore, foils with a specific hole size and arrangement permit a further automation of electron microscopic (EM) data acquisition procedures.