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Journal Article

Carboxylated photoswitchable diarylethenes for biolabeling and super-resolution RESOLFT microscopy.

MPS-Authors
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Roubinet,  B.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

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Bossi,  M. L.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

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Alt,  P.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

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Leutenegger,  M.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

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Shojaei,  H.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

/persons/resource/persons196937

Schnorrenberg,  S.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

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Nizamov,  S.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

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Belov,  V. N.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

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Hell,  S. W.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

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Fulltext (public)

2354743.pdf
(Publisher version), 3MB

Supplementary Material (public)

2354743_Suppl.pdf
(Supplementary material), 5MB

Citation

Roubinet, B., Bossi, M. L., Alt, P., Leutenegger, M., Shojaei, H., Schnorrenberg, S., et al. (2016). Carboxylated photoswitchable diarylethenes for biolabeling and super-resolution RESOLFT microscopy. Angewandte Chemie International Edition, 55(49), 15429-15433. doi:10.1002/anie.201607940.


Cite as: http://hdl.handle.net/11858/00-001M-0000-002B-AC7B-5
Abstract
Reversibly photoswitchable 1,2-bis(2-ethyl-6-phenyl-1-benzothiophene-1,1-dioxide-3-yl)perfluorocyclopentenes (EBT) having fluorescent "closed" forms were decorated with four or eight carboxylic groups and attached to antibodies. Low aggregation, efficient photoswitching in aqueous buffers, specific staining of cellular structures, and good photophysical properties were demonstrated. Alternating light pulses of UV and blue light induce numerous reversible photochemical transformations between two stables states with distinct structures. Using relatively low light intensities, EBTs were applied in biology-related super-resolution microscopy based on the reversible saturable (switchable) optical linear fluorescence transitions (RESOLFT) and demonstrated optical resolution of 75 nm.