Functional expression of the calcium release channel from skeletal muscle 
ryanodine receptor cDNA

Penner, R.; Neher, E.; Takeshima, H.; Nishimura, S.; Numa, S.

doi:10.1016/0014-5793(89)81532-7

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Functional expression of the calcium release channel from skeletal muscle ryanodine receptor cDNA

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Penner, R.
Department of Membrane Biophysics, MPI for biophysical chemistry, Max Planck Society;

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Neher, E.
Department of Membrane Biophysics, MPI for biophysical chemistry, Max Planck Society;

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Citation

Penner, R., Neher, E., Takeshima, H., Nishimura, S., & Numa, S. (1989). Functional expression of the calcium release channel from skeletal muscle ryanodine receptor cDNA. FEBS Letters, 259(1), 217-221. doi:10.1016/0014-5793(89)81532-7.

Cite as: https://hdl.handle.net/11858/00-001M-0000-002B-B79B-A

Abstract

Combined patch-clamp and fura-2 measurements were performed to study the calcium release properties of Chinese hamster ovary (CHO) cells transfected with the rabbit skeletal muscle ryanodine receptor cDNA carried by an expression vector. Both caffeine (1–50 mM) and ryanodine (100 μM) induced release of calcium from intracellular stores of transformed CHO cells but not from control (non-transfected) CHO cells. The calcium responses to caffeine and ryanodine closely resembled those commonly observed in skeletal muscle. Repetitive applications of caffeine produced characteristic all-or-none rises in intracellular calcium. Inositol 1,4,5-trisphosphate (IP3) neither activated the ryanodine receptor channel nor interfered with the caffeine-elicited calcium release. These results indicate that functional calcium release channels are formed by expression of the ryanodine receptor cDNA.