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Adaptive wavefront correction in two-photon microscopy using coherence gated wavefront sensing

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Rückel,  Markus
Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society;

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Mack-Bucher,  Julia Angela
Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society;

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Denk,  Winfried
Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society;

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Citation

Rückel, M., Mack-Bucher, J. A., & Denk, W. (2006). Adaptive wavefront correction in two-photon microscopy using coherence gated wavefront sensing. Proceedings of the National Academy of Sciences of the United States of America (ASTOR), 103(46), 17137-17142. doi:10.1073/pnas.0604791103.


Cite as: http://hdl.handle.net/11858/00-001M-0000-002B-BC90-9
Abstract
The image quality of a two-photon microscope is often degraded by wavefront aberrations induced by the specimen. We demonstrate here that resolution and signal size in two-photon microcopy can be substantially improved, even in living biological specimens, by adaptive wavefront correction based on sensing the wavefront of coherence-gated backscattered light (coherence-gated wavefront sensing, CGWS) and wavefront control by a deformable mirror. A nearly diffraction-limited focus can be restored even for strong aberrations. CGWS-based wavefront correction should be applicable to samples with a wide range of scattering properties and it should be possible to perform real-time pixel-by-pixel correction even at fast scan speeds.