On the fundamental imaging-depth limit in two-photon microsopy

Theer, Patrick; Denk, Winfried

doi:10.1364/JOSAA.23.003139

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On the fundamental imaging-depth limit in two-photon microsopy

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Theer, Patrick
Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society;

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Denk, Winfried
Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society;

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https://www.osapublishing.org/josaa/viewmedia.cfm?uri=josaa-23-12-3139&seq=0
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Citation

Theer, P., & Denk, W. (2006). On the fundamental imaging-depth limit in two-photon microsopy. Journal of the Optical Society of America. A, Optics, image science, and vision, 23(12), 3139-3149. doi:10.1364/JOSAA.23.003139.

Cite as: https://hdl.handle.net/11858/00-001M-0000-002B-BDBD-0

Abstract

We have analyzed how the maximal imaging depth of two-photon microscopy in scattering samples depends on properties of the sample and the imaging system. We find that the imaging depth increases with increasing numerical aperture and staining inhomogeneity and with decreasing excitation-pulse duration and scattering anisotropy factor, but is ultimately limited by near-surface fluorescence with slight improvements possible using special detection strategies.