Single-shot diffraction data from the Mimivirus particle using an X-ray 
free-electron laser.

Ekeberg, T.; Svenda, M.; Seibert, M. M.; Abergel, C.; Maia, F. R. N. C.; Seltzer, V.; DePonte, D. P.; Aquila, A.; Andreasson, J.; Iwan, B.; Jönsson, O.; Westphal, D.; Odic, D.; Andersson, I.; Barty, A.; Liang, M.; Martin, A. V.; Gumprecht, L.; Fleckenstein, H.; Bajt, S.; Barthelmess, M.; Coppola, N.; Claverie, J.-M.; Loh, N. D.; Bostedt, C.; Bozek, J. D.; Krzywinski, J.; Messerschmidt, M.; Bogan, M. J.; Hampton, C. Y.; Sierra, R. G.; Frank, M.; Shoeman, R. L.; Lomb, L.; Foucar, L.; Epp, S. W.; Rolles, D.; Rudenko, A.; Hartmann, R.; Hartmann, A.; Kimmel, N.; Holl, P.; Weidenspointner, G.; Rudek, B.; Erk, B.; Kassemeyer, S.; Schlichting, I.; Strüder, L.; Ullrich, J.; Schmidt, C.; Krasniqi, F.; Hauser, G.; Reich, C.; Soltau, H.; Schorb, S.; Hirsemann, H.; Wunderer, C.; Graafsma, H.; Chapman, H.; Hajdu, J.

doi:10.1038/sdata.2016.60

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Single-shot diffraction data from the Mimivirus particle using an X-ray free-electron laser.

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Rolles, D.
Research Group of Structural Dynamics of (Bio)Chemical Systems, MPI for Biophysical Chemistry, Max Planck Society;

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Citation

Ekeberg, T., Svenda, M., Seibert, M. M., Abergel, C., Maia, F. R. N. C., Seltzer, V., et al. (2016). Single-shot diffraction data from the Mimivirus particle using an X-ray free-electron laser. Scientific Data, 3: 160060. doi:10.1038/sdata.2016.60.

Cite as: https://hdl.handle.net/11858/00-001M-0000-002C-0D1E-6

Abstract

Free-electron lasers (FEL) hold the potential to revolutionize structural biology by producing X-ray pules short enough to outrun radiation damage, thus allowing imaging of biological samples without the limitation from radiation damage. Thus, a major part of the scientific case for the first FELs was three-dimensional (3D) reconstruction of non-crystalline biological objects. In a recent publication we demonstrated the first 3D reconstruction of a biological object from an X-ray FEL using this technique. The sample was the giant Mimivirus, which is one of the largest known viruses with a diameter of 450 nm. Here we present the dataset used for this successful reconstruction. Data-analysis methods for single-particle imaging at FELs are undergoing heavy development but data collection relies on very limited time available through a highly competitive proposal process. This dataset provides experimental data to the entire community and could boost algorithm development and provide a benchmark dataset for new algorithms.