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Journal Article

Three-dimensional reconstruction of the giant mimivirus particle with an X-ray free-electron laser.

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Rolles,  D.
Research Group of Structural Dynamics of (Bio)Chemical Systems, MPI for Biophysical Chemistry, Max Planck Society;

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2367251.pdf
(Publisher version), 2MB

Supplementary Material (public)

2367251_Suppl.pdf
(Supplementary material), 227KB

Citation

Ekeberg, T., Svenda, M., Abergel, C., Maia, F. R. N. C., Seltzer, V., Claverie, J.-M., et al. (2015). Three-dimensional reconstruction of the giant mimivirus particle with an X-ray free-electron laser. Physical Review Letters, 114(9): 098102. doi:10.1103/PhysRevLett.114.098102.


Cite as: http://hdl.handle.net/11858/00-001M-0000-002C-0DAB-8
Abstract
We present a proof-of-concept three-dimensional reconstruction of the giant mimivirus particle from experimentally measured diffraction patterns from an x-ray free-electron laser. Three-dimensional imaging requires the assembly of many two-dimensional patterns into an internally consistent Fourier volume. Since each particle is randomly oriented when exposed to the x-ray pulse, relative orientations have to be retrieved from the diffraction data alone. We achieve this with a modified version of the expand, maximize and compress algorithm and validate our result using new methods.