Fluorescent Arc/Arg3.1 indicator mice: a versatile tool to study brain activity 
changes in vitro and in vivo

Grinevich, Valery; Kolleker, Aleksandre; Eliava, Marina; Takada, Naoki; Takuma, Hiroshi; Fukuzawa, Y.; Shigemoto, Ryuichi; Kuhl, Dietmar; Waters, David Jack; Seeburg, Peter H.; Osten, Pavel

doi:10.1016/j.jneumeth.2009.07.015

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Fluorescent Arc/Arg3.1 indicator mice: a versatile tool to study brain activity changes in vitro and in vivo

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Grinevich, Valery
Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons93841

Kolleker, Aleksandre
Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons92795

Eliava, Marina
Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons95591

Takuma, Hiroshi
Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons95861

Waters, David Jack
Department of Cell Physiology, Max Planck Institute for Medical Research, Max Planck Society;

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Seeburg, Peter H.
Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons94635

Osten, Pavel
Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society;

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http://www.sciencedirect.com/science/article/pii/S0165027009003914/pdfft?md5=b23f41acd0fae6f99374d93dc24b841a&pid=1-s2.0-S0165027009003914-main.pdf
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http://dx.doi.org/10.1016/j.jneumeth.2009.07.015
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Citation

Grinevich, V., Kolleker, A., Eliava, M., Takada, N., Takuma, H., Fukuzawa, Y., et al. (2009). Fluorescent Arc/Arg3.1 indicator mice: a versatile tool to study brain activity changes in vitro and in vivo. Journal of Neuroscience Methods, 184(1), 25-26. doi:10.1016/j.jneumeth.2009.07.015.

Cite as: https://hdl.handle.net/11858/00-001M-0000-002C-110B-3

Abstract

The brain-specific immediate early gene Arc/Arg3.1 is induced in response to a variety of stimuli, including sensory and behavior-linked neural activity. Here we report the generation of transgenic mice, termed TgArc/Arg3.1-d4EGFP, expressing a 4-h half-life form of enhanced green fluorescent protein (d4EGFP) under the control of the Arc/Arg3.1 promoter. We show that d4EGFP-mediated fluorescence faithfully reports Arc/Arg3.1 induction in response to physiological, pathological and pharmacological stimuli, and that this fluorescence permits electrical recording from activated neurons in the live mouse. Moreover, the fluorescent Arc/Arg3.1 indicator revealed activity changes in circumscribed brain areas in distinct modes of stress and in a mouse model of Alzheimer's disease. These findings identify the TgArc/Arg3.1-d4EGFP mouse as a versatile tool to monitor Arc/Arg3.1 induction in neural circuits, both in vitro and in vivo.