Functional characterization of the DnaK chaperone system from the archaeon 
Methanothermobacter thermautotrophicus ΔH

Popp, Simone; Reinstein, Jochen

doi:10.1016/j.febslet.2008.12.062

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Functional characterization of the DnaK chaperone system from the archaeon Methanothermobacter thermautotrophicus ΔH

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Popp, Simone
Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society;

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Reinstein, Jochen
Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society;

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Citation

Popp, S., & Reinstein, J. (2009). Functional characterization of the DnaK chaperone system from the archaeon Methanothermobacter thermautotrophicus ΔH. FEBS Letters, 583(3), 573-578. doi:10.1016/j.febslet.2008.12.062.

Cite as: https://hdl.handle.net/11858/00-001M-0000-002C-1234-0

Abstract

We characterized the biochemical and functional properties of the DnaK system from the archaeon Methanothermobacter thermautotrophicus ΔH. In contrast to the eubacterial chaperone components the archaeal Hsp70 system shows thermal transitions only slightly above the optimal environmental temperature (65 °C). Nevertheless, it prevents aggregation of luciferase in the physiological temperature range of the organism, but is also fully functional at 30 °C in luciferase refolding. Additionally, GrpE_M.th. and DnaJ_M.th. substitute their eubacterial counterparts whereas DnaK_M.th. is only functional with its native cochaperones which could be attributed to a functional specialization of the eubacterial chaperones during evolution.