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Causal interrogation of neuronal networks and behavior through virally transduced ivermectin receptors

MPG-Autoren
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Obenhaus,  Horst A.
Rolf Sprengel Group, Max Planck Institute for Medical Research, Max Planck Society;

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Bertocchi,  Ilaria
Rolf Sprengel Group, Max Planck Institute for Medical Research, Max Planck Society;

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Tang,  Wannan
Rolf Sprengel Group, Max Planck Institute for Medical Research, Max Planck Society;

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Betz,  Heinrich
Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society;

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Sprengel,  Rolf
Rolf Sprengel Group, Max Planck Institute for Medical Research, Max Planck Society;
Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society;

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Zitation

Obenhaus, H. A., Rozov, A., Bertocchi, I., Tang, W., Kirsch, J., Betz, H., et al. (2016). Causal interrogation of neuronal networks and behavior through virally transduced ivermectin receptors. Frontiers in Molecular Neuroscience, 9: 75, pp. 1-17. doi:10.3389/fnmol.2016.00075.


Zitierlink: http://hdl.handle.net/11858/00-001M-0000-002C-150E-0
Zusammenfassung
The causal interrogation of neuronal networks involved in specific behaviors requires the spatially and temporally controlled modulation of neuronal activity. For long-term manipulation of neuronal activity, chemogenetic tools provide a reasonable alternative to short-term optogenetic approaches. Here we show that virus mediated gene transfer of the ivermectin (IVM) activated glycine receptor mutant GlyRα1 (AG) can be used for the selective and reversible silencing of specific neuronal networks in mice. In the striatum, dorsal hippocampus, and olfactory bulb, GlyRα1 (AG) promoted IVM dependent effects in representative behavioral assays. Moreover, GlyRα1 (AG) mediated silencing had a strong and reversible impact on neuronal ensemble activity and c-Fos activation in the olfactory bulb. Together our results demonstrate that long-term, reversible and re-inducible neuronal silencing via GlyRα1 (AG) is a promising tool for the interrogation of network mechanisms underlying the control of behavior and memory formation.