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Microconstriction Arrays for High-Throughput Quantitative Measurements of Cell Mechanical Properties

MPG-Autoren
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Harder,  Irina
Optical Design and Microoptics, Leuchs Division, Max Planck Institute for the Science of Light, Max Planck Society;

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Zitation

Lange, J. R., Steinwachs, J., Kolb, T., Lautscham, L. A., Harder, I., Whyte, G., et al. (2015). Microconstriction Arrays for High-Throughput Quantitative Measurements of Cell Mechanical Properties. BIOPHYSICAL JOURNAL, 109(1), 26-34. doi:10.1016/j.bpj.2015.05.029.


Zitierlink: http://hdl.handle.net/11858/00-001M-0000-002D-63A0-3
Zusammenfassung
We describe a method for quantifying the mechanical properties of cells in suspension with a microfluidic device consisting of a parallel array of micron-sized constrictions. Using a high-speed charge-coupled device camera, we measure the flow speed, cell deformation, and entry time into the constrictions of several hundred cells per minute during their passage through the device. From the flow speed and the occupation state of the microconstriction array with cells, the driving pressure across each constriction is continuously computed. Cell entry times into microconstrictions decrease with increased driving pressure and decreased cell size according to a power law. From this power-law relationship, the cell elasticity and fluidity can be estimated. When cells are treated with drugs that depolymerize or stabilize the cytoskeleton or the nucleus, elasticity and fluidity data from all treatments collapse onto a master curve. Power-law rheology and collapse onto a master curve are predicted by the theory of soft glassy materials and have been previously shown to describe the mechanical behavior of cells adhering to a substrate. Our finding that this theory also applies to cells in suspension provides the foundation for a quantitative high-throughput measurement of cell mechanical properties with microfluidic devices.