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Journal Article

Cryogenic Colocalization Microscopy for Nanometer-Distance Measurements

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Weisenburger,  Siegfried
Sandoghdar Division, Max Planck Institute for the Science of Light, Max Planck Society;

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Sandoghdar,  Vahid
Sandoghdar Division, Max Planck Institute for the Science of Light, Max Planck Society;

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Citation

Weisenburger, S., Jing, B., Haenni, D., Reymond, L., Schuler, B., Renn, A., et al. (2014). Cryogenic Colocalization Microscopy for Nanometer-Distance Measurements. SI, 15(4), 763-770. doi:10.1002/cphc.201301080.


Cite as: http://hdl.handle.net/11858/00-001M-0000-002D-6627-E
Abstract
The main limiting factor in spatial resolution of localization microscopy is the number of detected photons. Recently we showed that cryogenic measurements improve the photostability of fluorophores, giving access to Angstrom precision in localization of single molecules. Here, we extend this method to colocalize two fluorophores attached to well-defined positions of a double-stranded DNA. By measuring the separations of the fluorophore pairs prepared at different design positions, we verify the feasibility of cryogenic distance measurement with sub-nanometer accuracy. We discuss the important challenges of our method as well as its potential for further improvement and various applications.