English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
Add to Basket
  Local TagsRelease HistoryDetailsSummary

Released
Journal Article

Cryogenic Colocalization Microscopy for Nanometer-Distance Measurements

MPS-Authors
/persons/resource/persons201230

Weisenburger,  Siegfried
Sandoghdar Division, Max Planck Institute for the Science of Light, Max Planck Society;

/persons/resource/persons201175

Sandoghdar,  Vahid
Sandoghdar Division, Max Planck Institute for the Science of Light, Max Planck Society;

External Resource
No external resources are shared
Fulltext (restricted access)
There are currently no full texts shared for your IP range.
Fulltext (public)
There are no public fulltexts stored in PuRe
Supplementary Material (public)
There is no public supplementary material available
Citation

Weisenburger, S., Jing, B., Haenni, D., Reymond, L., Schuler, B., Renn, A., et al. (2014). Cryogenic Colocalization Microscopy for Nanometer-Distance Measurements. SI, 15, 763-770. doi:10.1002/cphc.201301080.


Cite as: https://hdl.handle.net/11858/00-001M-0000-002D-6627-E
Abstract
The main limiting factor in spatial resolution of localization microscopy is the number of detected photons. Recently we showed that cryogenic measurements improve the photostability of fluorophores, giving access to Angstrom precision in localization of single molecules. Here, we extend this method to colocalize two fluorophores attached to well-defined positions of a double-stranded DNA. By measuring the separations of the fluorophore pairs prepared at different design positions, we verify the feasibility of cryogenic distance measurement with sub-nanometer accuracy. We discuss the important challenges of our method as well as its potential for further improvement and various applications.