Minimal synthetic cells to study integrin-mediated adhesion

Frohnmayer, Johannes; Brüggemann, Dorothea; Eberhard, Christian; Neubauer, Stefanie; Mollenhauer, Christine; Böhm, Heike; Kessler, Horst; Geiger, Benjamin; Spatz, Joachim P.

doi:10.1002/anie.201503184

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Minimal synthetic cells to study integrin-mediated adhesion

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Frohnmayer, Johannes
Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society;
Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany;

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Brüggemann, Dorothea
Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society;

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Eberhard, Christian
Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society;
Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany;

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Mollenhauer, Christine
Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society;
Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany;

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Böhm, Heike
Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society;
Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany;

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Spatz, Joachim P.
Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society;
Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany;

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Citation

Frohnmayer, J., Brüggemann, D., Eberhard, C., Neubauer, S., Mollenhauer, C., Böhm, H., et al. (2015). Minimal synthetic cells to study integrin-mediated adhesion. Angewandte Chemie International Edition in English, 54(42), 12472-12478. doi:10.1002/anie.201503184.

Cite as: https://hdl.handle.net/11858/00-001M-0000-0028-DBB0-E

Abstract

To shed light on cell-adhesion-related molecular pathways, synthetic cells offer the unique advantage of a well-controlled model system with reduced molecular complexity. Herein, we show that liposomes with the reconstituted platelet integrin αIIb β3 as the adhesion-mediating transmembrane protein are a functional minimal cell model for studying cellular adhesion mechanisms in a defined environment. The interaction of these synthetic cells with various extracellular matrix proteins was analyzed using a quartz crystal microbalance with dissipation monitoring. The data indicated that integrin was functionally incorporated into the lipid vesicles, thus enabling integrin-specific adhesion of the engineered liposomes to fibrinogen- and fibronectin-functionalized surfaces. Then, we were able to initiate the detachment of integrin liposomes from these surfaces in the presence of the peptide GRGDSP, a process that is even faster with our newly synthesized peptide mimetic SN529, which specifically inhibits the integrin αIIb β3 .