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Nanopore diameters tune strain in extruded fibronectin fibers

MPG-Autoren
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Raoufi,  Mohammad
Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society;
Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany;

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Das,  Tamal
Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society;
Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany;

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Brüggemann,  Dorothea
Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society;

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Spatz,  Joachim P.
Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society;
Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany;

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Zitation

Raoufi, M., Das, T., Schoen, I., Vogel, V., Brüggemann, D., & Spatz, J. P. (2015). Nanopore diameters tune strain in extruded fibronectin fibers. Nano Letters, 15(10), 6357-6364. doi:10.1021/acs.nanolett.5b01356.


Zitierlink: http://hdl.handle.net/11858/00-001M-0000-0028-DAE8-9
Zusammenfassung
Fibronectin is present in the extracellular matrix and can be assembled into nanofibers in vivo by undergoing conformational changes. Here, we present a novel approach to prepare fibronectin nanofibers under physiological conditions using an extrusion approach through nanoporous aluminum oxide membranes. This one-step process can prepare nanofiber bundles up to a millimeter in length and with uniform fiber diameters in the nanometer range. Most importantly, by using different pore diameters and protein concentrations in the extrusion process, we could induce varying lasting structural changes in the fibers, which were monitored by Förster resonance energy transfer and should impose different physiological functions.