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Nanoparticle tension probes patterned at the nanoscale: impact of integrin clustering on force transmission

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Medda,  Rebecca
Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society;
Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany;

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Spatz,  Joachim P.
Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society;
Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany;

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Cavalcanti-Adam,  Elisabetta Ada
Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society;
Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany;

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Citation

Liu, Y., Medda, R., Liu, Z., Galior, K., Yehl, K., Spatz, J. P., et al. (2014). Nanoparticle tension probes patterned at the nanoscale: impact of integrin clustering on force transmission. Nano Letters, 14(10), 5539-5546. doi:10.1021/nl501912g.


Cite as: http://hdl.handle.net/11858/00-001M-0000-0024-30DF-0
Abstract
Herein we aimed to understand how nanoscale clustering of RGD ligands alters the mechano-regulation of their integrin receptors. We combined molecular tension fluorescence microscopy with block copolymer micelle nanolithography to fabricate substrates with arrays of precisely spaced probes that can generate a 10-fold fluorescence response to pN-forces. We found that the mechanism of sensing ligand spacing is force-mediated. This strategy is broadly applicable to investigating receptor clustering and its role in mechanotransduction pathways.