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Cobalt(III) as a stable and inert mediator ion between NTA and His6- tagged proteins

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Wegner,  Seraphine V.
Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society;
Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany;

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Spatz,  Joachim P.
Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society;
Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany;

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Citation

Wegner, S. V., & Spatz, J. P. (2013). Cobalt(III) as a stable and inert mediator ion between NTA and His6- tagged proteins. Angewandte Chemie International Edition: a journal of the Gesellschaft Deutscher Chemiker, 52(29), 7593-7596. doi:10.1002/anie.201210317.


Cite as: http://hdl.handle.net/11858/00-001M-0000-0014-C690-D
Abstract
Don't let go! The Co(3+)-mediated interaction between nitrilotriacetic acid (NTA) and the His6-tag is so stable and inert towards ligand exchange that it has a half-life of 7 days in the presence of imidazole and survives even under strongly chelating as well as reducing conditions, unlike the commonly used Ni(2+) or Co(2+) complexes. Possible applications include the separation of labeled proteins and the stable immobilization of proteins on surfaces.