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Surface properties of nanostructured bio-active interfaces: impacts of surface stiffness and topography on cell–surface interactions

MPG-Autoren
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Platzman,  Ilia
Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society;
Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany;

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Muth,  Christine A.
Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society;

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Lee-Thedieck,  Cornelia
Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society;

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Pallarola,  Diego
Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society;
Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany;

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Louban,  Ilia
Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society;

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Altrock,  Eva
Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society;

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Spatz,  Joachim P.
Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society;
Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany;

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Zitation

Platzman, I., Muth, C. A., Lee-Thedieck, C., Pallarola, D., Atanasova, R., Louban, I., et al. (2013). Surface properties of nanostructured bio-active interfaces: impacts of surface stiffness and topography on cell–surface interactions. RSC Advances, 3(32), 13293-13303. doi:10.1039/C3RA41579A.


Zitierlink: http://hdl.handle.net/11858/00-001M-0000-0014-C602-D
Zusammenfassung
Due to their ability to confer key functions of the native extracellular matrix (ECM), poly(ethylene glycol) (PEG)-based and PEG-modified materials have been extensively used as biocompatible and bio-functionalized substrate systems to study the influence of environmental parameters on cell adhesion in vitro. Given wide-ranging recent evidence that ECM compliance influences a variety of cell functions, detailed determination and characterization of the specific PEG surface characteristics including topography, stiffness and chemistry is required. Here, we studied two frequently used bio-active interfaces—PEG-based and PEG-modified surfaces—to elucidate the differences between the physical surface properties, which cells can sense and respond to. For this purpose, two sets of surfaces were synthesized: the first set consisted of nanopatterned glass surfaces containing cRGD-functionalized gold nanoparticles surrounded by a passivated PEG-silane layer and the second set consisted of PEG-diacrylate (PEG-DA) hydrogels decorated with cRGD-functionalized gold nanoparticles. Although the two sets of nanostructured materials compared here were highly similar in terms of density and geometrical distribution of the presented bio-ligands, as well as in terms of mechanical bulk properties, the topography and mechanical properties of the surfaces were found to be substantially different and are described in detail. In comparison to the very stiff and ultra-smooth surface properties of the PEG-passivated glasses, the mechanical properties of PEG-DA surfaces in the biologically relevant stiffness range, together with the increased surface roughness at micro- and nanoscale levels have the potential to affect cell behavior. This potential was verified by studying the adhesive behavior of hematopoietic KG-1a and rat embryonic fibroblast (REF52) cells on both surfaces.