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Journal Article

Cyclic stretch increases splicing noise rate in cultured human fibroblasts

MPS-Authors
/persons/resource/persons75390

Deibler,  Martin
Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society;

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Spatz,  Joachim P.
Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society;
Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany;

/persons/resource/persons75667

Kemkemer,  Ralf
Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society;

External Resource

http://bmcresnotes.biomedcentral.com/track/pdf/10.1186/1756-0500-4-470?site=bmcresnotes.biomedcentral.com
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https://dx.doi.org/10.1186/1756-0500-4-470
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Citation

Uhl, M., Mellert, K., Striegl, B., Deibler, M., Lamla, M., Spatz, J. P., et al. (2011). Cyclic stretch increases splicing noise rate in cultured human fibroblasts. BMC Research Notes, 4: 470, pp. 1-7. doi:10.1186/1756-0500-4-470.


Cite as: https://hdl.handle.net/11858/00-001M-0000-0010-4E6A-B
Abstract
BACKGROUND: Mechanical forces are known to alter the expression of genes, but it has so far not been reported whether they may influence the fidelity of nucleus-based processes. One experimental approach permitting to address this question is the application of cyclic stretch to cultured human fibroblasts. As a marker for the precision of nucleus-based processes, the number of errors that occur during co-transcriptional splicing can then be measured. This so-called splicing noise is found at low frequency in pre-mRNA splicing. FINDINGS: The amount of splicing noise was measured by RT-qPCR of seven exon skips from the test genes AATF, MAP3K11, NF1, PCGF2, POLR2A and RABAC1. In cells treated by altered uniaxial cyclic stretching for 18 h, a uniform and significant increase of splicing noise was found for all detectable exon skips. CONCLUSION: Our data demonstrate that application of cyclic stretch to cultured fibroblasts correlates with a reduced transcriptional fidelity caused by increasing splicing noise.