English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT

Released

Journal Article

Kinetic fingerprint discriminates similar cell populations subjected to uniaxial cyclic tensile strain on flexible substrates

MPS-Authors
/persons/resource/persons75390

Deibler,  Martin
Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons76081

Schulz,  Simon
Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons75667

Kemkemer,  Ralf
Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society;

Fulltext (restricted access)
There are currently no full texts shared for your IP range.
Fulltext (public)
There are no public fulltexts stored in PuRe
Supplementary Material (public)
There is no public supplementary material available
Citation

Woertche, E., Deibler, M., Schulz, S., Steinberg, T., Kemkemer, R., & Tomakidi, P. (2011). Kinetic fingerprint discriminates similar cell populations subjected to uniaxial cyclic tensile strain on flexible substrates. Soft Matter, 7, 8612-8618. doi:10.1039/c1sm05551h.


Cite as: http://hdl.handle.net/11858/00-001M-0000-0010-4E14-9
Abstract
Uniaxial cyclic tensile deformation of flexible cell culture membranes has been reported to induce cell body and cytoskeleton alignment with respect to the axis of the applied mechanical stimulus. This effect was proven to be strongly dependent on the stretching frequency and amplitude. However there is only little information available on cell type-specific responses. Extension of this stretching technique by live-cell imaging allowed us to investigate the temporal behavior of cells, which enabled us to define a cell-specific kinetic fingerprint. Based on this approach, we could identify significant differences between two periodontal cell populations closely related in vivo. The orientation response was shown to be faster for periodontal ligament fibroblasts than for alveolar bone cells. These results were substantiated by our observation of altered activation levels of RhoA. With this work we could show that the combination of biomechanical stimulation and live cell imaging provides a potential tool to distinguish between morphologically and biochemically very similar cell populations and to reveal altered stimuli-dependent physiological processes, as indicated by the kinetic fingerprint on flexible substrates. Moreover, these new findings can be implemented in the development of cell type-/tissue-specific customized substrates based on soft matter biomaterials or prospective smart flexible polymers.