Biomimetic models of the actin cortex

Haraszti, Tamas; Schulz, Simon; Uhrig, Kai; Kurre, Rainer; Roos, Wouter; Schmitz, Christian H. J.; Curtis, Jennifer E.; Maier, Timo; Clemen, Anabel E.-M.; Spatz, Joachim P.

doi:10.1142/S1793048009001009

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Biomimetic models of the actin cortex

MPG-Autoren

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Haraszti, Tamas
Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society;
Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany;

/persons/resource/persons76081

Schulz, Simon
Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons76214

Uhrig, Kai
Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons76010

Roos, Wouter
Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons75378

Curtis, Jennifer E.
Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons75812

Maier, Timo
Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society;
Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany;

/persons/resource/persons75370

Clemen, Anabel E.-M.
Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons76135

Spatz, Joachim P.
Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society;
Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany;

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http://www.worldscientific.com/doi/pdf/10.1142/S1793048009001009
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http://dx.doi.org/10.1142/S1793048009001009
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Zitation

Haraszti, T., Schulz, S., Uhrig, K., Kurre, R., Roos, W., Schmitz, C. H. J., et al. (2009). Biomimetic models of the actin cortex. Biophysical Reviews and Letters, 4(1&2), 17-32. doi:10.1142/S1793048009001009.

Zitierlink: http://hdl.handle.net/11858/00-001M-0000-0010-3DEE-C

Zusammenfassung

The cytoskeleton is an actively regulated complex network in the cell. One of the most researched components is actin. In our work we developed and tested two microfluidic systems both being applicable to construct quasi 2-dimensional biomimetic actin networks. The first system uses polydimethylsiloxane micropillars, the other polystyrene microparticles held by holographic optical tweezers as anchoring points. Our devices provide actin networks with mesh sizes from a few micrometers up to the order of 10 micrometers. Qualitative analysis shows similar network formation in both systems. Crosslinking was tested using filamin, α-actinin, Ca and Mg ions. The crosslinking process is characterized by a zipping like event, which is limited only by the high stretching modulus of the actin filaments.