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A quantitative 3D motility analysis of trypanosoma brucei by use of digital in-line holographic microscopy

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Maier,  Timo
Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society;
Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany;

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Haraszti,  Tamás
Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society;
Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany;

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Grunze,  Michael
Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society;

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Citation

Weiße, S., Heddergott, N., Heydt, M., Pflästerer, D., Maier, T., Haraszti, T., et al. (2012). A quantitative 3D motility analysis of trypanosoma brucei by use of digital in-line holographic microscopy. PLoS One, 7(5): e37296, pp. 1-13. doi:10.1371/journal.pone.0037296.


Cite as: http://hdl.handle.net/11858/00-001M-0000-0010-4B92-7
Abstract
We present a quantitative 3D analysis of the motility of the blood parasite Trypanosoma brucei. Digital in-line holographic microscopy has been used to track single cells with high temporal and spatial accuracy to obtain quantitative data on their behavior. Comparing bloodstream form and insect form trypanosomes as well as mutant and wildtype cells under varying external conditions we were able to derive a general two-state-run-and-tumble-model for trypanosome motility. Differences in the motility of distinct strains indicate that adaption of the trypanosomes to their natural environments involves a change in their mode of swimming.