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Continuous photobleaching to study the growth modes of focal adhesions

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Majer,  Günter
Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society;

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Spatz,  Joachim P.
Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society;
Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany;

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Citation

de Beer, A. G. F., Majer, G., Roke, S., & Spatz, J. P. (2010). Continuous photobleaching to study the growth modes of focal adhesions. Journal of Adhesion Science and Technology, 24(13-14), 2323-2334. doi:10.1163/016942410X508046.


Cite as: http://hdl.handle.net/11858/00-001M-0000-0010-75D7-2
Abstract
We combine total internal reflection fluorescence microscopy with continuous photobleaching to study the growth mode of focal adhesions in rat embryonic fibroblasts (REF-52). We measured GFP-labelled β3 integrin, which exhibits a diffusion rate of 10–14–10–13 m2/s when it is not bound to a focal adhesion. We show that exchange of β3 integrin between focal adhesions and the surrounding membrane occurs only at the edges of the focal adhesion. We, therefore, conclude that focal adhesions show an edge-on/edge-off growth, in contrast to the bulk-on/bulk-off growth mode that is assumed in many theoretical treatments.