Deutsch
 
Benutzerhandbuch Datenschutzhinweis Impressum Kontakt
  DetailsucheBrowse

Datensatz

DATENSATZ AKTIONENEXPORT

Freigegeben

Zeitschriftenartikel

Geometric organization of the extracellular matrix in the control of integrin-mediated adhesion and cell function in osteoblasts of the alveolar bone

MPG-Autoren
/persons/resource/persons75354

Cavalcanti-Adam,  Elisabetta Ada
Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society;
Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany;

/persons/resource/persons76135

Spatz,  Joachim P.
Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society;
Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany;

Externe Ressourcen
Es sind keine Externen Ressourcen verfügbar
Volltexte (frei zugänglich)
Es sind keine frei zugänglichen Volltexte verfügbar
Ergänzendes Material (frei zugänglich)
Es sind keine frei zugänglichen Ergänzenden Materialien verfügbar
Zitation

Cavalcanti-Adam, E. A., Tomakidi, P., Bezler, M., & Spatz, J. P. (2005). Geometric organization of the extracellular matrix in the control of integrin-mediated adhesion and cell function in osteoblasts of the alveolar bone. Progress Orthodontics, 6(2), 232-237. Retrieved from https://www.ncbi.nlm.nih.gov/labs/articles/16276432/.


Zitierlink: http://hdl.handle.net/11858/00-001M-0000-0010-4953-0
Zusammenfassung
Cell-extracellular matrix (ECM) interactions play a central role in tissue architecture and turnover. Particularly, integrin-mediated cell adhesion participates in biochemical and physical signals. The aim of this study is to investigate the importance of ECM organization for alveolar bone osteoblasts adhesion and to determine the effects on cell functions such as collagen and fibronectin production. By applying new concepts from the nanotechnology to biological systems, we have developed materials decorated with nano-patterns of peptides of the ECM arranged at a distance of 58 or 73 nm. On these surfaces, human osteoblasts from alveolar bone were cultured for 1-96 hr and examined by video and fluorescence microscopy. Protein quantification by western blotting and gene expression by RT-PCR were also performed. Good cell adhesion and spreading was observed on the 58 nm pattern after 30 min, while weak adhesion and increased motility was evident in osteoblasts on the 73 nm pattern, leading to alteration of cell shape and reduction of cell area after 24 hr. Moreover, cells on the 73 nm did not form focal adhesions and failed to organize the cytoskeleton. After 96 hr in culture, osteoblasts on the 73 nm retained intracellular collagen and produced a disorganized fibronectin network. Osteoblast adhesion and intra-and extra-cellular molecules reorganization are regulated not only by the composition but also by the structure of the extracellular environment. Our novel in vitro system makes it possible to elucidate some of the mechanisms necessary for the maintenance of tissue architecture and mechanical strength, as well as for the design of artificial materials for future clinical applications.