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Membrane-grafted hyaluronan films: a well-defined model system of glycoconjugate cell coats

MPG-Autoren
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Böhm,  Heike
Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society;
Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany;

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Spatz,  Joachim P.
Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society;
Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany;

Externe Ressourcen

http://pubs.acs.org/doi/pdf/10.1021/ja068768s
(beliebiger Volltext)

https://dx.doi.org/10.1021/ja068768s
(beliebiger Volltext)

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Zitation

Richter, R. P., Hock, K. K., Burkhartsmeyer, J., Böhm, H., Bingen, P., Wang, G., et al. (2007). Membrane-grafted hyaluronan films: a well-defined model system of glycoconjugate cell coats. Journal of the American Chemical Society, 129(17), 5306-5307. doi:10.1021/ja068768s.


Zitierlink: https://hdl.handle.net/11858/00-001M-0000-0010-44E9-8
Zusammenfassung
We report on the creation of films of end-grafted hyaluronan, based on solid-supported lipid membranes. We characterize the layer thickness, the grafting density, the mechanical properties and the permeability of these highly hydrated and up to several hundred nanometer-thick monomolecular layers by quartz crystal microbalance with dissipation monitoring, by colloidal probe triple-wavelength reflection interference contrast microscopy, and by reflectometry. The two-dimensional assemblies thus created are expected to serve as versatile platforms to study, in a well-controlled and quantitative manner, the effect of hyaluronan-binding proteins on the structure, properties, and biological function of this type of films.